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VOLUME 14 , ISSUE 4 ( October, 2010 ) > List of Articles


Prevalence of metallo-β-lactamase producing Pseudomonas aeruginosa and Acinetobacter species in intensive care areas in a tertiary care hospital

Anuradha De, Simit Kumar, Sujata Baveja

Keywords : MBL producers, ICUs.

Citation Information : De A, Kumar S, Baveja S. Prevalence of metallo-β-lactamase producing Pseudomonas aeruginosa and Acinetobacter species in intensive care areas in a tertiary care hospital. Indian J Crit Care Med 2010; 14 (4):217-219.

DOI: 10.4103/0972-5229.76089

License: CC BY-ND 3.0

Published Online: 01-03-2018

Copyright Statement:  Copyright © 2010; The Author(s).


A total of 39 non-duplicate isolates of carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter species isolated from blood and endotracheal secretions were tested for metallo-β-lactamase (MBL) production by modified-EDTA disc synergy and double disc synergy tests. The prevalence of MBLs was 33.33% by both the above tests. All patients with MBL-positive isolates were multidrug resistant and had multiple risk factors like > 8 days hospital stay, catheterization, IV lines, previous antibiotic use, etc. These were risk factors for imipenem resistance also. The overall mortality in MBL-positive patients was 46.15%.

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  1. Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-β-lactamases: The quiet before the storm? Clin Microbiol Rev 2005;18:306-25.
  2. Butt T, Usman M, Ahmed RN, Saif I. Emergence of Metallo- β-lactamase producing Pseudomonas aeruginosa in Pakistan. J Pak Med Assoc 2005;55:302-4.
  3. Pitout JDD, Gregson DB, Poirel L, McClure JA, Le P, Church DL. Detection of Pseudomonas aeruginosa producing Metallo-β-lactamases in a large centralized laboratory. J Clin Microbiol 2005;43:3129-35.
  4. Marra AR, Pereira CA, Gales AC, Menezes LC, Cal RG, de Souza JM, et al. Blood stream infections with Metallo-β-lactamase producing Pseudomonas aeruginosa: Epidemiology, microbiology and clinical outcomes. Antimicrob Agents Chemother 2006;50:388-90.
  5. Behera B, Mathur P, Das A, Kapil A, Sharma V. An evaluation of four different phenotypic techniques for detection of Metallo-β-lactamase producing Pseudomonas aeruginosa. Indian J Med Microbiol 2008;26:233-7.
  6. Jayakumar S, Appalaraju B. Prevalence of multi and pan drug resistant Pseudomonas aeruginosa with respect to ESBL and MBL in a tertiary care hospital. Indian J Pathol Microbiol 2007;50:922-5.
  7. Shanthi M, Sekar U. Multi-drug resistant Pseudomonas aeruginosa and Acinetobacter baumanii infections among hospitalized patients: Risk factors and outcomes. J Assoc Phys India 2009;57:636-45.
  8. Varaiya A, Kulkarni A, Kulkarni M, Bhalekar P, Dogra J. Incidence of Metallo-beta-lactamase producing Pseudomonas aeruginosa in ICU patients. Indian J Med Res 2008;127:398-402.
  9. Lee K, Chong Y, Shin HB, Kim YA, Yong D, Yum JH. Modified Hodge and EDTA-disk synergy tests to screen Metallo-b-lactamase strains of Pseudomonas and Acinetobacter species. Clin Microbiol Infect Dis 2001;7:88-91.
  10. Lee K, Lim YS, Yong D, Yum JH, Chong Y. Evaluation of the Hodge Test and the imipenem-EDTA Double Disk Synergy Test for differentiating Metallo-β-lactamase producing isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol 2003;41:4623-9.
  11. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. Clinical Laboratory Standards Institute. 2007; M100-S17;27: p. 38-41.
  12. Yong D, Choi YS, Roh KH, Kim CK, Park YH, Yum JH, et al. Increasing prevalence and diversity of Metallo-β-lactamases in Pseudomonas spp., Acinetobacter spp. and Enterobacteriaceae from Korea. Antimicrob Agents Chemother 2006;50:1884-6.
  13. Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA. Global challenge of multidrug-resistant Acinetobacter baumanii. Antimicrob Agents Chemother 2007;51:3471-84.
  14. Organisms with Metallo-beta-lactamases (MBLs). Infection Control Fact Sheet 2007. Infection Control and Hospital Epidemiology Unit, Alfred Hospital. 2007. Available from: [last accessed on 2008 Sep 18].
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