Citation Information :
Prashant A, Vishwanath P, Kotekar N, Doddamani P, Nataraj SM. Can soluble CD163 predict outcome of patients with acute respiratory distress from mechanical ventilation?: A pilot study. Indian J Crit Care Med 2013; 17 (6):355-358.
Purpose: CD163 is a monocyte/macrophage-associated antigen which has recently been identified as a hemoglobin scavenger receptor and has also anti-inflammatory properties and an immunoregulatory role. This surface receptor undergoes ectodomain shedding upon an inflammatory stimulus, leading to increased fraction of soluble CD163 (sCD163). Hence, we hypothesized that the mechanical ventilation (MV) which is known to elicit inflammatory response may cause increased serum levels of sCD163 which can predict the outcome of patients from MV. Subjects and Methods: Thirty patients with acute respiratory distress aged >18 years who required MV were enrolled for the study. Serum levels of sCD163 were estimated using quantitative immunometric sandwich enzyme immunoassay technique from 3 mL of the venous blood sample which was collected immediately and at 24 h after the patient was connected to MV. On the basis of the outcome of the patient from MV, they were divided into two groups; survivors and nonsurvivors. Results: Out of the 30 patients, 18 patients survived and 12 patients expired. Serum levels of sCD163 were significantly increased in nonsurvivors when compared with survivors (P < 0.01) at 24 h after connecting to MV. sCD163 > 1020 ng/mL at 24 h of MV increases the probability of mortality by factor 6. An increase of sCD163 by 1 ng/mL significantly increases the relative probability of mortality by a factor of 1.0017 (95% confidence interval, 1.0004-1.0030, P = 0.0005). Conclusions: Elevated levels of sCD163 at 24 h of MV help in predicting the outcome of patients with acute respiratory failure from MV.
Póvoa P, Almeida E, Moreira P, Fernandes A, Mealha R, Aragão A, et al. C-reactive protein as an indicator of sepsis. Intensive Care Med 1998;24:1052-6.
Bhattacharya B, Prashant A, Vishwanath P, Suma MN, Nataraj B. Prediction of outcome and prognosis of patients on mechanical ventilation using body mass index, SOFA score, C-reactive proteins and serum albumin. Indian J Crit Care Med 2011;15:82-7.
Högger P, Dreier J, Droste A, Buck F, Sorg C. Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163). J Immunol 1998;161:1883-90.
Kristiansen M, Graversen JH, Jacobsen C, Sonne O, Hoffman HJ, Law SK, et al. Identification of the haemoglobin scavenger receptor. Nature 2001;409:198-201.
Zuwa³a-Jagie³³o J. Haemoglobin scavenger receptor: Function in relation to disease. Acta Biochim Pol 2006;53:257-68.
Davis BH, Zarev PV. Human monocyte CD163 expression inversely correlates with soluble CD163 plasma levels. Cytometry B Clin Cytom 2005;63:16-22.
Graversen JH, Madsen M, Moestrup SK. CD163: A signal receptor scavenging haptoglobin-haemoglobin complexes from plasma. Int J Biochem Cell Biol 2002;34:309-14.
Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, et al. CD antigens 1996. Blood 1997;89:3502.
Zwadlo G, Voegeli R, Schulze Osthoff K, Sorg C. A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp Cell Biol 1987;55:295-304.
Schaer DJ, Boretti FS, Hongegger A, Poehler D, Linnscheid P, Staege H, et al. Molecular cloning and characterization of the mouse CD163 homologue a highly glucocorticoid-inducible member of the scavenger receptor cysteine-rich family. Immunogenetics 2001;53:170-7.
Müller HJ, de Fost M, Aerts H, Hollak C, Moestrup SK. Plasma level of the macrophage-derived soluble CD163 is increased and positively correlates with severity in Gaucher′s disease. Eur J Haematol 2004;72:135-9.
Matsushita N, Kashiwagi M, Wait R, Nagayoshi R, Nakamura M, Matsuda T, et al. Elevated levels of soluble CD163 in sera and fluids from rheumatoid arthritis patients and inhibition of the shedding of CD163 by TIMP-3. Clin Exp Immunol 2002;130:156-61.
Baeten D, Müller HJ, Delanghe J, Veys EM, Moestrup SK, De Keyser F. Association of CD163+macrophages and local production of soluble CD163 with decreased lymphocyte activation in spondylarthropathy synovitis. Arthritis Rheum 2004;50:1611-23.
Groselj-Grenc M, Ihan A, Derganc M. Neutrophil and monocyte CD64 and CD163 expression in critically ill neonates and children with sepsis: Comparison of fluorescence intensities and calculated indexes. Mediators Inflamm 2008;2008:202646.