Indian Journal of Critical Care Medicine

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 20 , ISSUE 9 ( 2016 ) > List of Articles

BRIEF COMMUNICATION

Comparative evaluation of the role of single and multiple blood specimens in the outcome of blood cultures using BacT/ALERT 3D (automated) blood culture system in a tertiary care hospital

Prithwis Bhattacharyya, Himesh Barman, D. Elantamilan, Valarie Lyngdoh, Annie B. Khyriem, Jyotismita Rajbongshi, Ishani Bora, Surbala Devi

Keywords : BacT/ALERT 3D, bacteremia, blood stream infections, FA plus, number of samples

Citation Information : Bhattacharyya P, Barman H, Elantamilan D, Lyngdoh V, Khyriem AB, Rajbongshi J, Bora I, Devi S. Comparative evaluation of the role of single and multiple blood specimens in the outcome of blood cultures using BacT/ALERT 3D (automated) blood culture system in a tertiary care hospital. Indian J Crit Care Med 2016; 20 (9):530-533.

DOI: 10.5005/ijccm-20-9-530

License: CC BY-ND 3.0

Published Online: 00-09-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction: Bloodstream infection (BSI) is a leading cause of mortality in critically ill patients. The mortality directly attributable to BSI has been estimated to be around 16% and 40% in general hospital population and Intensive Care Unit (ICU) population, respectively. The detection rate of these infections increases with the number of blood samples obtained for culture. The newer continuous monitoring automated blood culture systems with enhanced culture media show increased yield and sensitivity. Hence, we aimed at studying the role of single and multiple blood specimens from different sites at the same time in the outcome of automated blood culture system. Materials and Methods and Results: A total of 1054 blood culture sets were analyzed over 1 year, the sensitivity of one, two, and three samples in a set was found to be 85.67%, 96.59%, and 100%, respectively, which showed a statistically significant difference (P < 0.0001). Similar findings were seen in few more studies, however, among individual organisms in contrast to other studies, the isolation rates of Gram-positive bacteria were less than that of Gram-negative Bacilli with one (or first) sample in a blood culture set. In our study, despite using BacT/ALERT three-dimensional continuous culture monitoring system with FAN plus culture bottles, 15% of positive cultures would have been missed if only a single sample was collected in a blood culture set. Conclusion: The variables like the volume of blood and number of samples collected from different sites still play a major role in the outcome of these automated blood culture systems.


PDF Share
  1. Seifert H, Wisplinghoff H. Bloodstream infection and endocarditis. In: Mahy BW, ter Meulen V, Borriello SP, Murray PR, Funke G, Kaufmann SH, et al., editors. Topley and Wilson's Microbiology and Microbial Infections. Chichester, UK: John Wiley and Sons, Ltd.; 2010.
  2. Kirn TJ, Mirrett S, Reller LB, Weinstein MP. Controlled clinical comparison of BacT/alert FA plus and FN plus blood culture media with BacT/alert FA and FN blood culture media. J Clin Microbiol 2014;52:839-43.
  3. Lamy B, Roy P, Carret G, Flandrois JP, Delignette-Muller ML. What is the relevance of obtaining multiple blood samples for culture? A comprehensive model to optimize the strategy for diagnosing bacteremia. Clin Infect Dis 2002;35:842-50.
  4. Weinstein MP, Murphy JR, Reller LB, Lichtenstein KA. The clinical significance of positive blood cultures: A comprehensive analysis of 500 episodes of bacteremia and fungemia in adults. II. Clinical observations with special reference to factors influencing prognosis. Rev Infect Dis. 1983;5:54-70.
  5. Washington JA 2nd. Blood cultures: Principles and techniques. Mayo Clin Proc 1975;50:91-8.
  6. Cockerill FR 3rd, Wilson JW, Vetter EA, Goodman KM, Torgerson CA, Harmsen WS, et al. Optimal testing parameters for blood cultures. Clin Infect Dis 2004;38:1724-30.
  7. Lee A, Mirrett S, Reller LB, Weinstein MP. Detection of bloodstream infections in adults: How many blood cultures are needed? J Clin Microbiol 2007;45:3546-8.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.