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VOLUME 16 , ISSUE 3 ( July, 2012 ) > List of Articles


Comparative efficacy evaluation of disinfectants routinely used in hospital practice: India

Malkit Singh, Neelam Taneja, Meera Sharma, Pramod K. Gupta, Jatinder K Rana

Keywords : Disinfectant, evaluation, hospital practice

Citation Information : Singh M, Taneja N, Sharma M, Gupta PK, Rana JK. Comparative efficacy evaluation of disinfectants routinely used in hospital practice: India. Indian J Crit Care Med 2012; 16 (3):123-129.

DOI: 10.4103/0972-5229.102067

License: CC BY-ND 3.0

Published Online: 01-04-2018

Copyright Statement:  Copyright © 2012; The Author(s).


Aim: The aim of this study was to evaluate and compare practically achieved disinfection efficacy of some locally available disinfectants on surfaces and infectious microbiological hospital waste. Materials and Methods: Seven disinfectants were tested at concentrations recommended by manufacturers on rough and smooth surfaces that were contaminated experimentally by locally circulating isolates of methicillin-resistant Staphylococcus aureus, multidrug-resistant Acinetobacter baumannii, Klebsiella pneumoniae, Enterobacter aerogenes, Pseudomonas aeruginosa strains, standard isolate of Salmonella typhi and Candida albicans. Reduction in microbial counts before and after surface disinfection was expressed as log reduction. A very heavy microbial waste load was simulated by immersing culture plates with heavy microbial growth in disinfectants. Daily, a sample of disinfectant was taken and subjected to in-use test. Results: The highest average log reduction of test microbes on the rough surface was given by DesNet (5.05) and Bacillocid special (5.02). A comparable average log reduction of test microbes on a smooth steel surface was noted (5.68, 5.67, 5.50) for Lysol, Bacillocid sp. and DesNet, respectively. In the discard jars, Bacillocid special worked satisfactorily for 4 days, DesNet for 3 days and Hi-giene Germitol for 1 day. The remainder of the disinfectants failed in the in-use test on Day 1. Phenolics, although widely used in our settings, may not be as good surface disinfectants as newer formulations like DesNet and Bacillocid special. Conclusions: Newer quaternary ammonium compounds and aldehyde formulations were found to be the best disinfectants for disinfection of heavy contamination.

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  1. BSG Working party Report. 2003. Available from: [Last accessed]Accessed on April 01, 2008
  2. The standardisation of disinfectants. J R Sanit Inst 1903;24:424-41.
  3. A study of the Chick- Martin test for disinfectants. J Hyg (Lond) 1934;34:322-32.
  4. A new test for the assessment of disinfectants with particular reference to their use in hospitals. Pharm J 1969;202:607-9.
  5. An improved Kelsey-Sykes test for disinfectants. Pharm J 1974;213:528-30.
  6. Reduction of disinfectant bactericidal activities in clinically isolated Acinetobacter spp in the presence of organic material. J Antimicrob Chemother 2008;61:568-76.
  7. Do European Standard Disinfectant tests truly simulate in-use microbial and organic soiling conditions on food preparation surfaces? J Appl Microbiol 2010;108:1344-51.
  8. Standard Quantitative Disk Carrier Test Method for determining the bactericidal, virucidal, fungicidal, mycobactericidal and sporicidal activities of liquid chemical germicides. Active Standard ASTM E2197.Developed by Subcommittee: E35.15, Book of Standards Volume: 11.05.
  9. Surface disinfection: Should we do it? J Hosp Infect 2001;48:S64-8.
  10. Comparison of the effect of detergent versus hypochlorite cleaning on environmental contamination and incidence of Clostridium difficile infection. J Hosp Infect 2003;54:109-14.
  11. A microbiological evaluation of hospital cleaning methods. Int J Environ Health Res 2007;17:285-95.
  12. APIC guidelines for selection and use of disinfectants. Am J Infect Control 1996;24:313-42.
  13. A prospective study of nosocomial infections in burn patients at a tertiary care centre in north India. Burns 2004;30:665-9.
  14. Hospital environment contamination with gram negative bacteria is as common and important as that with gram-positive bacteria in an overloaded tertiary care hospital in India. J Hosp Infect 2005;59:164-5.
  15. Hydrogen peroxide fogging in an overcrowded tertiary care center: Some practical queries. J Hosp Infect 2005;60:85.
  16. Environmental contamination due to methicillin-resistant Staphylococcus aureus (MRSA). J Hosp Infect 1998;38:67-70.
  17. Evidence that hospital hygiene is important in the control of methicillin resistant Staphyloccus aureus. J Hosp Infect 2001;49:109-16.
  18. Correlation between reduced susceptibility to disinfectants and multi drug resistance among clinical isolates of Acinetobacter spp. J Antimicrob Chemother 2010;65:1973-83.
  19. Choosing a disinfectant for hospital environment. Indoor Environment Connections. 2009;10. Available from: [Last accessed date]
  20. Definition of terms. In: Block SS, editor. Disinfection sterilization and prevention, 4 th ed. Philadelphia, PA: Lea and Febiger; 1991. p. 18-25.
  21. Glutaraldehyde-induced and formaldehyde-induced allergic contact dermatitis among dental hygienists and assistants. J Am Dent Assoc 2003;134:1072-8.
  22. Occupational asthma due to glutaraldehyde and formaldehyde in endoscopy and x ray departments. Thorax 1995;50:156-9.
  23. Safe disposal of infectious waste -Indian perspective. J Hosp Infect 2006;62:525-7.
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